Superovulation, in vitro fertilization (IVF) and in vitro development (IVD) protocols for inbred BALB/cJ mice in comparison with outbred NMRI mice
نویسندگان
چکیده
منابع مشابه
Evaluation of different strain mice during in vitro fertilization and embryo development in response to superovulation
Background: Mice are the most commonly used animal in reproductive research and following the urgent need for these type of studies and also due to the increased interest in the ethical principle of animal rights, four various inbred and outbred strains of the laboratory mouse were evaluated to select the more efficient one for reproductive research. Materials and methods: 60 female and 16 mal...
متن کاملEffect of Taurine and Curcumin on In-Vitro Fertilization in NMRI Mice Treated with Acrylamide
Introduction: The aim of this study was to evaluate the effect of taurine and curcumin on in vitro fertilization (IVF) in female mice treated with acrylamideand and its effect on malondialdehyde (MDA) concentration and total antioxidant capacity (TAC). Methods: A total of 48 adult female NMRI mice were randomly divided into 6 groups, including control, curcumin 200 mg / kg, taurine 150 mg / kg...
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Background Oxidative stress induced by cyclophosphamide (CP), as a chemotherapeutic agent, causes vulnerability of sperm and declining the fertility capacity. Then in this study the effect of crocin (saffron extract) on amelioration of these side effects was investigated. MaterialsAndMethods In this study 24 mice were divided in three groups (n=8). Control group received normal saline (0.2 ml/d...
متن کاملSuperovulation, fertilization and in vitro embryo development in mice after administration of an inhibin-neutralizing antiserum.
The present study was conducted to investigate the effect of immunoneutralization against endogenous inhibin on oocyte and embryo production in adult and immature mice. At 12:00 h on day 2 of oestrus (day 1 of dioestrus), a single i.p. injection of inhibin antiserum (50, 100, 200 or 400 microl per animal) or equine chorionic gonadotrophin (eCG; 10 or 20 iu per animal) or control goat serum (100...
متن کاملP-141: Comparison Study The Effect of In Vitro Maturation and IVF Fertilization of Cryopreserved Oocyte with Fresh Oocyte in Mice
Background: Cryobiology and in vitro maturation of oocytes are very important tools of reproductive technology. Oocyte plays an important role in fertility. The objective of this research is comparison study the effect of invitro maturation and IVF fertilization of cryopreserved oocyte with fresh oocyte in mice. Materials and Methods: For this purpose, after superovulation was performed oocytes...
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ژورنال
عنوان ژورنال: Reproductive Medicine and Biology
سال: 2012
ISSN: 1445-5781
DOI: 10.1007/s12522-012-0127-8